Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...
Gene expression experiments measure changes in RNA transcript levels to compare how genes are up- or down-regulated across ...
A highly efficient and reliable fluorescent probe for PCR DNA amplification techniques and DNA analysis in hybridization experiments has been developed. This technology will enable the development of ...
Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...
Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...
CARLSBAD, Calif.--(BUSINESS WIRE)--Researchers can now simultaneously analyze samples for multiple genetic markers of interest in a single qPCR reaction with a set of new Life Technologies TaqMan ...
Expert Rev Mol Diagn. 2012;12(7):731-754. SYBR Green and dual-hybridization probes are often used for melting point analysis at the end of the amplification. qPCR presents a number of advantages over ...
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