SOLVED: A student loaded a mixture of compounds for column chromatographic separation in 1 mL of Ethanol, then proceeded to elute with Hexane: Ethanol (6:4). All of the mixture came off in the first 4 fractions, with no separation. What technical mistake had the student made?Why is it necessary to perform a thin layer chromatography (TLC) before carrying out the Column chromatography?Define the following: a/ Eluent. b/ Elute. c/ Elution.
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